METHODS FOR QUANTITATING HIGH-DENSITY LIPOPROTEIN CHOLESTEROL

摘要

Methods for quantitating HDL cholesterol, comprising adding to serum a surfactant selected from among polyoxyethylene alkylene phenyl ethers and polyoxyethylene alkylene tribenzylphenyl ethers and an enzyme reagent for assaying cholesterol, optionally together with a substance capable of inhibiting the reaction of cholesterol in serum lipoproteins with the above enzyme reagent, and determining the amount of the cholesterol thus reacted within a period of time where cholesterol in HDL, among lipoproteins, preferentially reacts with the above enzyme reagent. By using this method, cholesterol in HDL can be conveniently and efficiently quantitated without resort to any pretreatment such as centrifugation. Thus, it is applicable to various automatic analyzers.