发明名称 |
PROCESSO DE PREPARACAO DE UM MEIO DE CULTURA DE CELULAS DE MAMIFERO, ISENTO DE SORO, A BASE DE MEIO BASAL, LEVEDURA HIDROLISADA, DEXTRANO OU ALBUMINA, INSULINATRANSFERRINA, FRUTOSE FERRICA. CITRATO E SULFATO FERROSO E UM COMPONENTE DE ACIDO GORDO |
摘要 |
<p>The invention provides serum-free media for the culture of mammalian cells comprising a synthetic basal medium designed for mammalian cell culture; about 0.1 to about 10 grams per liter hydrolyzed yeast; about 0.1 to about 5 grams per liter of dextran or albumin; about 2 to about 20 milligrams per liter insulin; 0 to about 100 milligrams per liter of a compound selected from the group consisting of transferrin, ferric fructose, ferrous citrate and ferrous sulfate; and a fatty acid component consisting of oleic acid, linoleic acid and linolenic acid in a ratio of about 0.6: 1: 0.14 milligrams of fatty acid per liter.</p> |
申请公布号 |
PT99048(B) |
申请公布日期 |
1999.02.26 |
申请号 |
PT19910099048 |
申请日期 |
1991.09.25 |
申请人 |
SMITHKLINE BEECHAM CORPORATION |
发明人 |
LUCIANO RAMOS;AMY ANNE MURNANE;MELVIN SUSUMU OKA |
分类号 |
C12N5/06;C12N5/00;(IPC1-7):C12N5/06;C12N15/12;C12N5/10 |
主分类号 |
C12N5/06 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|