| 摘要 |
<p>A technique for gene expression specific to cells free from a specific transcription factor, which comprises constructing a recombinase expression unit wherein a recombinase gene is located in the downstream of a promoter depending on the specific transcription factor and another unit wherein two target sequences of a desired gene to be expressed and the recombinase are located in the downstream of another promoter; and infecting cells with these units. According to this technique, in cells carrying the specific transcription factor, the recombinase is expressed and thus recombination occurs between the recognition sequences, whereby the target gene is not expressed but deleted. In cells free from the specific transcription factor, on the other hand, the recombinase is not expressed and thus no recombination occurs between the recognition sequences, which allows the expression of the target gene.</p> |
