| 摘要 |
<p>A process and apparatus in which cryoprotectant in cryopreserved samples is removed with the use of turbulence and convective-dispersion around the cells within the primary cryopreservation container are described. Convective-dispersion is applied via the combined action of gravity and pulsative transmembrane flow of buffer through the pores of containers fabricated from appropriate membrane material. Use of mechanical forces to aid the process of cryoprotectant removal permits the benefits of slow cryoprotectant removal to be retained, while the actual removal time is decreased.</p> |
