Microbiological process for the preparation of (S,S)-N,N'-ethylenediaminedisuccinic acid

摘要

PCT No. PCT/EP96/01868 Sec. 371 Date Jan. 23, 1998 Sec. 102(e) Date Jan. 23, 1998 PCT Filed May 4, 1996 PCT Pub. No. WO96/36725 PCT Pub. Date Nov. 21, 1996A microbiological process for the preparation of N,N'-ethylenediaminedisuccinic acid of formula (1), by (a) precultivation of a strain of the genus Amycolatopsis orientalis on a specified nutrient medium (1st preculture), (b) inoculation of a nutrient solution of the same composition as the base of the production fermenter by the 1st preculture (2nd preculture), (c) inoculation of the base nutrient solution in the production fermenter with the 2nd preculture and incubation in accordance with a specified timetable, (d) feeding of the production fermenter in the fed-batch process with a feeding solution under aerobic conditions at a pH of from 2 to 8 and at a temperature of from 15 DEG to 40 DEG C., wherein the base nutrient and feeding solutions are free of zinc-containing compounds, is described. The compound prepared by the process according to the invention is suitable as complexing agent with good biodegradability in detergents. (1)