摘要 |
A process for producing genetically modified plant material comprising one or more stably incorporated DNA sequence(s) of interest, which comprises subjecting plant material that consists of or comprises shoots, shoot tips or apexes, rooted shoots or seedlings, to Agrobacterium-mediated transfer of the DNA sequence(s), the Agrobacterium-mediated DNA transfer being carried out in an oxygenated, for example, aerated liquid culture medium in which the plant material is cultivated, the plant material being submerged in the liquid medium. The medium is generally agitated or otherwise moving. The plant material is preferably actively growing in the submerged culture. |