发明名称 |
USE OF DNA ENCODING PLASTID PYRUVATE DEHYDROGENASE AND BRANCHED CHAIN OXOACID DEHYDROGENASE COMPONENTS TO ENHANCE POLYHYDROXYALKANOATE BIOSYNTHESIS IN PLANTS |
摘要 |
Provided are nucleic acid coding sequences and methods utilizing these sequences for optimizing levels of substrates employed in the biosynthesis of copolymers of 3-hydroxybutyrate (3HB) and 3-hydroxyvalerate (3HV) in plants via manipulation of normal metabolic pathways using recombinant techniques. This optimization is achieved through the use of a variety of wild-type and/or deregulated enzymes involved in the biosynthesis of aspartate family amino acids, and wild-type or deregulated forms of enzymes, such as threonine deaminase, involved in the conversion of threonine to P(3HB-co-3HV) copolymer endproduct. These enzymes are used in conjunction with the E1 alpha , E1 beta , E2, and E3 subunits of plastid pyruvate dehydrogenase complexes and branched chain oxoacid dehydrogenase complexes or mitochondrial dihydrolipoamide dehydrogenase E3 components to enhance the levels of threonine, 2-oxobutyrate ( alpha -keto-butyrate), propionate, propionyl-CoA, beta -ketovaleryl-CoA, and beta -hydroxyvaleryl-CoA. Also provided are methods for the biological production of P(3HB-co-3HV) copolymer in plants utilizing the enhanced levels of propionyl-CoA produced therein. Introduction into plants of an appropriate beta -ketothiolase, a beta -ketoacyl-CoA reductase, and a PHA synthase in combinations with the aforementioned enzymes will permit such plants to produce commercially useful amounts of P(3HB-co-3HV) copolymers. |
申请公布号 |
WO9900505(A1) |
申请公布日期 |
1999.01.07 |
申请号 |
WO1998US13406 |
申请日期 |
1998.06.30 |
申请人 |
UNIVERSITY OF MISSOURI |
发明人 |
RANDALL, DOUGLAS, R.;JOHNSTON, MARK, L.;MIERNYK, JAN, A.;LUETHY, MICHAEL, H.;MOONEY, BRIAN, P. |
分类号 |
C12N1/21;C12N9/02;C12N9/04;C12N9/10;C12N15/28;C12N15/29;C12N15/53;C12N15/82;C12P7/62 |
主分类号 |
C12N1/21 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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