发明名称 |
AQUEOUS IMMUNOLOGIC ADJUVANT COMPOSITIONS OF MONOPHOSPHORYL LIPID A |
摘要 |
An aqueous adjuvant composition comprising an attenuated lipid A derivative and a surfactant or surfactants enhances the immunological response in a warm blooded animal to a protein antigen. Attenuated lipid A derivatives useful according to the subject invention include monophosphoryl lipid A and 3-O-deacylated monophosphoryl lipid A. A surfactant or mixtures of surfactants are dissolved in a solvant. 1,2-Dipalmitoyl-sn-glycero-3-phosphocholine is a preferred surfactant. The dissolved surfactant is added to an attenuated lipid A derivative to obtain a mixture. The molar ratio of attenuated lipid A derivative to surfactant in the mixture is about 4:1. The solvent is evaporated and water is added to the resulting film. The suspension is sonicated in a 60 DEG C water bath until it becomes clear. Animals administered the adjuvant formulation exhibited increased antibody responses to a given antigen as well as displayed enhanced lymphocyte proliferative and cytotoxic T-lymphocyte responses. Intranasal administration of the aqueous adjuvant composition and an antigen stimulates the production of serum and mucosal secreted IgA. |
申请公布号 |
WO9843670(A3) |
申请公布日期 |
1998.12.30 |
申请号 |
WO1998US06528 |
申请日期 |
1998.04.01 |
申请人 |
RIBI IMMUNOCHEM RESEARCH, INC. |
发明人 |
CRANE, R., THOMAS |
分类号 |
A61K39/39;A61P31/00;C07H13/06 |
主分类号 |
A61K39/39 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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