| 摘要 |
<p>The present invention provides mammalian DNA sequences that display a high degree of sequence identity to their Saccharomyces cerevisae counterparts, RCE1 and AFC1. Specifically, complementary DNA (cDNA) sequences of the human and mouse RCE functional homologs are provide. Human cDNA sequences encoding proteins having a high degree of amino acid sequence identity to the yeast Afc1p protein are also provided. This invention is also directed to recombinant DNA molecules comprising the mammalian DNA sequences, DNA molecules and antisense RNA molecules which hybridize under stringent hybridization conditions to those DNA sequences, hosts transformed with their recombinant DNA molecules and protein expression products produced by culturing the transformed hosts. Antibodies directed against the protein expression products are also provided. Also provided are assays to identify inhibitors of one or more mammalian CAAX processing enzymes and kits for making the above products and performing the above assays. Finally, this invention provides pharmaceutical compositions comprising an inhibitor of a mammalian CAAX processing enzyme, and methods for treating a CAAX-protein mediated disease or disorder in a patient by administering such a pharmaceutical composition..</p> |
