Rapid detection of mutations in the p53 gene

摘要

The present invention relates to means for cleaving a nucleic acid cleavage structure in a site-specific manner. Enzymes, including 5' nucleases and 3' exonucleases, are used to screen for known and unknown mutations, including single base changes, in the human p53 gene. Methods are provided which allow for the identification of genetic mutations in the human p53 gene in a sample.