| 摘要 |
PCT No. PCT/JP94/00851 Sec. 371 Date Apr. 3, 1996 Sec. 102(e) Date Apr. 3, 1996 PCT Filed May 30, 1994 PCT Pub. No. WO94/28167 PCT Pub. Date Dec. 8, 1994The present invention relates to a method for quantitatively determining ionized calcium in a sample by using a phospholipase, which comprises carrying out an enzyme reaction with the phospholipase in a buffer comprising a nitrogen heterocycle-binding sulfonic acid having a pK ranging 6.6 to 7.6 or a salt thereof. According to the method of the invention, the amount of ionized calcium in a sample, such as serum, can be accurately determined.
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