beta -fructofuranosidase its preparation and uses

摘要

A beta -fructofuranosidase with a molecular weight of 49,000+/-5,000 daltons on SDS-PAGE, an isoelectric point of 4.6+/-0.5, an optimum pH of about 5.5-6.0, and an optimum temperature of about 50 DEG C. in the presence of calcium ion. The enzyme acts on saccharides with a beta -fructofuranosidic linkage and other substances including other saccharides, sugar alcohols, and alcohols to produce fructosyl-transferred saccharides in a relatively high yield.