| 摘要 |
The invention describes an improved stable reagent composition and method for the determination of hemoglobin (Hb) using whole blood samples and automated hematology analyzers, for example, the TECHNICON H~TM series of instruments as sold by the assignee hereof. The Hb reagent comprises an inorgani c cyanide salt, at least one surfactant and a base and has a final pH of about 10. 0 to about 11.2, preferably about 10.4 to about 11.2 or less and more preferably abou t 10.8 to about 11.2 or less. This reagent is intended for use as a universal Hb determination reagent for all of the instruments in the aforementioned series an d will provide accurate and precise results without accompanying interference from elevated numbers of white blood cells, especially as found in abnormal blood specimens, and without system-to-system variation in the results obtained. |
