| 摘要 |
<p>A raffinose is produced by the action of a raffinose synthetase having the following properties on sucrose and galactinol: (1) activity and substrate specificity: raffinose is produced from sucrose and galactinol; (2) optimal pH: about 6-8; (3) optimal temp.: about 35-40 °C; (4) molecular weight: (i) molecular weight as measured by gel filtration chromatography: about 75-95 kDa; (ii) molecular weight as measured by polyacrylamide gel electrophoresis (native PAGE): about 90-100 kDa; and (iii) molecular weight as measured by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) under reducing conditions: about 90-100 kDa; (5) inhibition: it is inhibited by iodoacetamide, N-ethylmaleimide, and myoinositol.</p> |
