| 摘要 |
A method for the qualitative or quantitative determination of antibodies (Ab) comprises: (a) reacting the Ab with a specific antigen (Ag) so that Ab-Ag complexes can form; (b) reacting the formed complexes with a protein denaturing agent (I) comprising urea-hydrogen peroxide, so that Ab with low affinity for the Ag are separated from the Ag, while Ab with strong affinity for the Ag remain in complex with the Ag, and (c) determining the amount of complex. Also claimed are: (1) a wash solution for the assay comprising urea-hydrogen peroxide, and (2) a sample diluent buffer comprising urea-hydrogen peroxide. |
