YEAST CELLS COMPRISING AT LEAST TWO COPIES OF A DESIRED GENE INTEGRATED INTO THE CHROMOSOMAL GENOME AT MORE THAN ONE NON-RIBOSOMAL RNA ENCODING DOMAIN, PARTICULARLY WITH KLUYVEROMYCES

摘要

The present invention provides for a yeast cell comprising at least two copies of a desired gene integrated into its chromosomal genome, wherein said genome comprises at least two DNA domains suitable for integration of one or more copies of said desired gene, which domains share substantial sequence homology and are non-ribosomal RNA encoding DNA domains, and wherein at least two of said substantially homologous non-ribosomal RNA encoding domains DNA domains have at least one copy of the said desired gene integrated. The invention also provides methods for making yeast cells according to the invention, as well as the use thereof for making a protein, a peptide or metabolite.