| 摘要 |
Improvements to the polymerase chain reaction (PCR), a process for in vitro enzy matic amplification of specific nucleic acid sequences, can be achieved by changing the way that PCR reagents are mixed and the enzymatic reaction is started and by the replacement of mineral oil, commonly used as a vapor barrier to minimize sol vent evaporation, by a grease or wax. The use of such mixtures allows for the delay of reagent mixing until the first heating ste p of a PCR amplification, thereby reducing the enzymatic generation of nonspecific products which occurs when a complete mixtur e of PCR reagents, with or without test sample, stands at room temperature or below. These mixtures increase the shelf-life of P CR reagents and increase protection of the laboratory environment against contamination by PCR product.
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