发明名称 |
Human breast epithelial cell type with stem cell and luminal epithelial cell characteristics |
摘要 |
Described is a substantially purified human breast epithelial cell (Type I HBEC) displaying the following characteristics: variable cell shape; smooth cell colony boundary; deficiency in gap junctional intercellular communication; positive expression of epithelial membrane antigen and keratin 18; negative expression of keratin 14, alpha 6 integrin and gap junction genes for connexins (Cx26, Cx32 and Cx43); growth promotion by fetal bovine serum; induction by cholera toxin to differentiate into Type II HBEC (prior art); and acquisition anchorage independent growth by Semian virus 40 transfection. Also described is a method of obtaining the above-identified epithelial cells comprising the steps of: a) development of a mixture of human breast epithelial cells from reduction mammoplasty tissues using the MSU-1 medium; b) eliminating stromal fibroblasts by a trypsin (0.002%) and ethylenediamine tetraacetic acid (0.02%) solution; c) separating Type I HBEC from Type II HBEC which attach on culture dishes earlier by collecting Type I HBEC that remain in suspension after trypsinization and prolonged incubation; d) the continuing culture of these cells in MSU-1 medium supplemented with fetal bovine serum, which inhibits the growth of Type II HBEC while promoting the growth of Type I HBEC, gives rise to Type I HBEC. Described also is a new defined medium (the MSU-1 medium) which supports the growth of both Type I and Type II human breast epithelial cells.
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申请公布号 |
US5814511(A) |
申请公布日期 |
1998.09.29 |
申请号 |
US19950558786 |
申请日期 |
1995.11.16 |
申请人 |
MICHIGAN STATE UNIVERSITY |
发明人 |
CHANG, CHIA-CHENG;TROSKO, JAMES E. |
分类号 |
C12N5/071;C12N5/074;(IPC1-7):C12N5/00 |
主分类号 |
C12N5/071 |
代理机构 |
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