| 摘要 |
The invention provides purified proteins, DNA sequences that code on expression therefore, and recombinant nucleic acid sequences, including hosts transformed with such sequences, for transforming coffee plants to reduce or eliminate the synthesis of caffeine. Coffee plant cells are transformed by means of transforming vectors that comprise a nucleic acid sequence, operably linked to a transcription promoter in an antisense or sense orientation, that codes on transcription for an RNA that has a length sufficient to interfere with the expression of a specific enzyme in the pathway for caffeine biosynthesis. Coffee plants regenerated from transformed coffee plant cells exhibit caffeine production that has been reduced by up to 98% compared with untransformed coffee plants.
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