发明名称 |
Compositions for enzyme catalyzed template-independent creation of phosphodiester bonds using protected nucleotides |
摘要 |
A method for the stepwise creation of phosphodiester bonds between desired nucleosides resulting in the synthesis of polynucleotides having a predetermined nucleotide sequence by preparing an initiation substrate containing a free and unmodified 3'-hydroxyl group; attaching a mononucleotide selected according to the order of the predetermined nucleotide sequence to the 3'-hydroxyl of the initiating substrate in a solution containing a catalytic amount of an enzyme capable of catalyzing the 5' to 3' phosphodiester linkage of the 5'-phosphate of the mononucleotide to the 3'-hydroxyl of the initiating substrate, wherein the mononucleotide contains a protected 3'-hydroxyl group, whereby the protected mononucleotide is covalently linked to the initiating substrate and further additions are hindered by the 3'-hydroxyl protecting group. Methods in which a mononucleotide immobilized on a solid support is added to a free polynucleotide chain are also disclosed.
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申请公布号 |
US5808045(A) |
申请公布日期 |
1998.09.15 |
申请号 |
US19950486897 |
申请日期 |
1995.06.07 |
申请人 |
HIATT, ANDREW C.;ROSE, FLOYD D. |
发明人 |
HIATT, ANDREW C.;ROSE, FLOYD |
分类号 |
C07H19/10;C07H19/20;C07H21/00;C12P19/30;(IPC1-7):C07H19/04 |
主分类号 |
C07H19/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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