摘要 |
PROBLEM TO BE SOLVED: To obtain a pure microorganism that efficiently produces fructosyl amine oxidase by transformation with a DNA having a base sequence that codes for the amino acid sequence of fructosyl amine oxidase having specific amino acid sequences. SOLUTION: This fructosyl amine oxidase-productive microorganism is obtained by transforming a microorganism with a DNA having a base sequence that codes for an amino acid sequence of fructosyl amine oxidase having amino acid sequences 1 to 440 in the amino acid sequence of the formula. The transformed microorganism belongs to Escherichia coli, such as Escherichia coli DH1.pFOD7 (FERM P-15943), and the fructosyl amine oxidase is preferably produced by culturing this transformed microorganism in a culture medium under aerobic condition, and collecting the fructosyl amine oxidase from the culture medium. |