发明名称 |
Protein complexes having factor VIII:C activity and production thereof |
摘要 |
Recombinant protein complexes having human Factor VIII:C activity are expressed in a eukaryotic host cell by transforming the host cell with first and second expression cassettes encoding a first polypeptide substantially homologous to human Factor VIII:C A domain and a second polypeptide substantially homologous to human Factor VIII:C C domain, respectively. In the present invention, the first polypeptide may be extended having at its C-terminal a human Factor VIII:C B domain N-terminal peptide, a polypeptide spacer of 3-40 amino acids, and a human Factor VIII:C B domain C-terminal peptide. Expression of the second polypeptide is improved by employing an alpha 1-antitrypsin signal sequence.
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申请公布号 |
US5789203(A) |
申请公布日期 |
1998.08.04 |
申请号 |
US19940266170 |
申请日期 |
1994.06.27 |
申请人 |
CHIRON CORPORATION;NOVO NORDISK A/S |
发明人 |
CHAPMAN, BARBARA;BURKE, RAE LYN;RASMUSSEN, MIRELLA EZBAN;MIKKELSON, JAN MOLLER |
分类号 |
A61K38/00;C07H21/04;C07K14/755;C07K14/775;C12N1/00;C12N5/10;C12N15/12;C12N15/63;C12N15/85;C12P21/02;C12P21/04;(IPC1-7):C07K14/755 |
主分类号 |
A61K38/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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