发明名称 |
Method for amplification and sequencing of nucleic acid polymers |
摘要 |
Amplification and sequencing of a selected region of a target nucleic acid polymer are be performed in a single vessel. The sample is added to an amplification mixture containing a thermally stable polymerase and nucleoside feedstocks. Chain terminating dideoxynucleosides are added either at the beginning of the amplification reaction or during the course of the amplification. A thermally stable polymerase which incorporates dideoxynucleotides into an extending oligonucleotide at a rate which is no less than about 0.4 times the rate of incorporation of deoxynucleosides can be used in the amplification mixture or added with the chain terminating nucleoside.
|
申请公布号 |
US5789168(A) |
申请公布日期 |
1998.08.04 |
申请号 |
US19960640672 |
申请日期 |
1996.05.01 |
申请人 |
VISIBLE GENETICS INC. |
发明人 |
LEUSHNER, JAMES;HUI, MAY;DUNN, JAMES M.;LARSON, MARINA T. |
分类号 |
B01L7/00;C12Q1/68;C12Q1/70;G01N35/00;(IPC1-7):C12Q1/68;C12P19/34 |
主分类号 |
B01L7/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|