发明名称 |
Transgenic non-human animals for producing heterologous antibodies |
摘要 |
The invention relates to transgenic non-human animals capable of producing heterologous antibodies and transgenic non-human animals having inactivated endogenous immunoglobulin genes. In one aspect of the invention, endogenous immunoglobulin genes are suppressed by antisense polynucleotides and/or by antiserum directed against endogenous immunoglobulins. Heterologous antibodies are encoded by immunoglobulin genes not normally found in the genome of that species of non-human animal. In one aspect of the invention, one or more transgenes containing sequences of unrearranged heterologous human immunoglobulin heavy chains are introduced into a non-human animal thereby forming a transgenic animal capable of functionally rearranging transgenic immunoglobulin sequences and producing a repertoire of antibodies of various isotypes encoded by human immunoglobulin genes. Such heterologous human antibodies are produced in B-cells which are thereafter immortalized, e.g., by fusing with an immortalizing cell line such as a myeloma or by manipulating such B-cells by other techniques to perpetuate a cell line capable of producing a monoclonal heterologous antibody. The invention also relates to heavy and light chain immunoglobulin transgenes for making such transgenic non-human animals as well as methods and vectors for disrupting endogenous immunoglobulin loci in the transgenic animal.
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申请公布号 |
US5789650(A) |
申请公布日期 |
1998.08.04 |
申请号 |
US19920853408 |
申请日期 |
1992.03.18 |
申请人 |
GENPHARM INTERNATIONAL, INC. |
发明人 |
LONBERG, NILS;KAY, ROBERT M. |
分类号 |
C07K16/00;(IPC1-7):C12N15/00;C07H19/04 |
主分类号 |
C07K16/00 |
代理机构 |
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