发明名称 |
Method of producing insulin-like growth factor-I (IGF-I) and insulin-like growth factor binding protein-3 (IGFBP-3) with correct folding and disulfide bonding |
摘要 |
The present invention provides a novel method for refolding insulin-like growth factor I (IGF-I) and insulin-like growth factor binding protein 3(IGFBP-3). The method involves mixing of IGF-I and IGFBP-3 together in a cofolding reaction. The inventive cofolding method results in substantially higher yields of correctly folded protein for both molecules and alters the kinetics of refolding. The method includes the production of correctly folded IGF-I, IGFBP-3, and/or IGF-I/IGFBP-3 complex.
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申请公布号 |
US5789547(A) |
申请公布日期 |
1998.08.04 |
申请号 |
US19950482271 |
申请日期 |
1995.06.07 |
申请人 |
CELTRIX PHARMACEUTICALS, INC. |
发明人 |
SOMMER, ANDREAS;OGAWA, YASUSHI;TAO, PEGGY |
分类号 |
C12N15/09;A61K38/00;A61K38/27;A61P43/00;C07K1/113;C07K14/47;C07K14/65;C12N15/12;C12P21/00;C12R1/91;(IPC1-7):C07K1/14;C07K14/435 |
主分类号 |
C12N15/09 |
代理机构 |
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主权项 |
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地址 |
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