THERMOSTABLE DNA POLYMERASE FROM BACILLUS PALLIDUS.

摘要

A novel type I DNA polimerase derived from Bacillus pallidus is provided. The gene encoding the polymerase has been cloned and expressed Characterization of the enzyme revealed, in addition to DNA polymerase activity. 5-4' exonuclease activity. Reverse transcripate activity, strand displacing activity and the ability to initiate polymerization at a nick in double-stranded Dna. In certain constructs the domain encoding the 5'-3' exonuclease activity is delected to provide an enzyme which is particularly useful for Strand Displacement Amplification.