| 摘要 |
<p>A method for determining ascorbic acid by quantitatively determining ascorbic acid in a sample by using an ascorbate oxidase (hereinafter referred to as 'ASOD') capable of catalyzing a reaction of ascorbic acid of reduced form with oxygen to produce ascorbic acid of oxidized form and hydrogen peroxide, characterized by simultaneously conducting a reaction of ascorbic acid of reduced form with oxygen to produce ascorbic acid of oxidized form and hydrogen peroxide and a reaction of the resultant hydrogen peroxide with a chromogen in the presence of a peroxidase to produce a dye in the same reaction system in an aqueous medium in the presence of ASOD and the formed dye quantitatively determining. A method for quantitatively determining total ascorbic acid by adding a reducing agent capable of reducing ascorbic acid of oxidized form to ascorbic acid of reduced form to the above aqueous medium to conduct an enzymatic reaction, whereby total ascorbic acid present in a low concentration can be quantitatively determined. Another method for quantitatively determining total ascorbic acid by reducing ascorbic acid of oxidized form in the sample with a reducing agent capable of reducing ascorbic acid of oxidized form to ascorbic acid of reduced form and conducting an enzymatic reaction in the presence of a compound capable of depriving the reducing agent of the reducing power. A reagent for quantitatively determining ascorbic acid and a kit for quantitatively determining ascorbic acid used for the above quantitative determination methods. The above methods can be applied also to the screening for a clinical test, can simultaneously determine a plurality of analytes, and are simple.</p> |
