摘要 |
The present invention relates to five synthetic peptides pertaining to the protein pre-M/M of the dengue-2 virus corresponding to the amino acid sequence 2-31, 45-67, 57-92, 69-93 and 103-124. The immune response to the peptides was evaluated in rats. Recombinant fusion proteins including regions of pre-M/M were constructed. The presence of epitopes for B cells of rats and human beings in the peptides of the protein pre-M/M of the dengue virus was verified. the peptides 3-31 and 103-124 induced neutralizing antibodies against the four serotypes of the dengue virus. The studies of lymphoproliferation with the peptides 57-92 and 3-31 demonstrated crossed recognition of T cells which are peptido-specific to the virus. The rats tested with the dengue2 virus have demonstrated a protection induced by the peptides 3-31, 57-92 and 69-93. Thus, the presence of sequential epitopes in the protein pre-M/M of the dengue-2 virus and the possible relevance of the same in the immune response against said flavivirus is demonstrated. The Spanish text is not needed.
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申请人 |
CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA;INSTITUTO DE MEDICINA TROPICAL "PEDRO KOURI" |
发明人 |
REYES ACOSTA, OSVALDO;RODRIGUEZ ROCHE, ROSMARI;ALVAREZ VERA, MAYLIN;PADRON PALOMARES, GABRIEL;PEREZ DIAZ, ANA BEATRIZ;MORIER DIAZ, LUIS;PUPO ANTUNEZ, MARITZA;PEREZ INSUITA, OMAIDA;GUILLEN NIETO, GERARDO ENRIQUE;PARDO LAZO, ORLANDO LUIS;GARAY PEREZ, HILDA ELISA;PELEGRINO MARTINEZ DE LA COTERA, JOSE LUIS;VAZQUEZ RAMUDO, SUSANA;GUZMAN TIRADO, GUADALUPE;CHINEA SANTIAGO, GLAY |