Method of isolating regulations of T cell activation

摘要

This invention provides a multi-step, high throughput primary screen to identify immune regulators of T cell activation for use as therapeutic agents. Compositions or compounds are screened for their ability to stimulate or inhibit the expression of a reporter gene operatively linked to specific transcriptional control sequences in T cells. A composition or compound identified as an immune stimulator or inhibitor by the primary screen of this invention can then be further characterized to determine the target molecule on which the composition or compound acts to regulate T cell activation and T cell activation-dependent processes such as apoptosis.