Compositions and methods to promote homologous recombination in eukaryotic cells and organisms

摘要

The invention concerns genes encoding recombinases that can be used to promote homologous recombination in eukaryotic cells and expression vectors that can be used to transiently express recombinases in target cells. One embodiment of the invention encompasses genetically engineered nucleic acids that encode a non-naturally occurring recombinase that causes a greater rate of recombination than does the naturally occurring recombinase. Recombinases from Ustilago maydis, Saccharomyces cerevisiae are specifically included in the invention.