发明名称 |
Fluorescent nucleotide analog hairpin formation for detection of nuclei c acid hybridization |
摘要 |
This invention provides methods and compositions for the detection of nucleic acid interactions with other nucleic acids or with proteins. The methods generally utilize a nucleic acid (e.g., an oligonucleotide) that contains one or more fluorescent nucleotide analogues. The fluorescence of the nucleotide analogues is quenched (reduced) when they are incorporated into the oligonucleotide. Alteration of the normal conformation of the oligonucleotide by hybridization (e.g. to form a loop) or by protein binding reduces and/or eliminates the quench thereby causing a detectable increase in fluorescence. |
申请公布号 |
AU7847798(A) |
申请公布日期 |
1998.07.03 |
申请号 |
AU19980078477 |
申请日期 |
1997.12.10 |
申请人 |
THE GOVERNMENT OF THE UNITED STATES OF AMERICA, REPRESENTED BY |
发明人 |
MARY HAWKINS |
分类号 |
C12Q1/68 |
主分类号 |
C12Q1/68 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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