发明名称 |
Sequences derived from the genome of the c hepatitis virus, and use thereof |
摘要 |
The present invention relates to a series of cDNA sequences derived from the hepatitis C virus, called CHV-Cu, and the expression of the antigenes coded by said sequences. The viral RNA of the CHV-Cu was extracted from the serum of a patient with non-A, non-B hepatitis. The cDNA was amplified by PCR and the fragments obtained were cloned and sequenced. The fragments which coded for antigenic polypeptides were expressed in yeast, thereby obtaining a multimer protein or a virus-like particle. The analysis by electronic microscopy showed a particle having a diameter comprised between 20 and 60 nm with a flotation density between 1.16 and 1.30 g/cm<3> in gradients of saccharose and cesium chloride. The invention may be applied to the medical field for the development of therapeutical and prophylactic vaccines, for the production of proteins having a diagnostic interest, for the development of polyclonal antibodies, natural or recombinant monoclonal antibodies and anti-viral agents. |
申请公布号 |
AU5397498(A) |
申请公布日期 |
1998.07.03 |
申请号 |
AU19980053974 |
申请日期 |
1997.12.12 |
申请人 |
CENTRO DE INGENIERIA GENETICA Y BIOTECNOLOGIA (CIGB) |
发明人 |
JUAN MORALES GRILLO;ARIEL VIIA RODRIGUEZ;CIRO GARCIA MARTINEZ;NELSON ACOSTA RIVERO;SANTIAGO DUEIAS CARRERA;ODALYS GARCIA CABRERA;IVIS GUERRA AIZPURUA;VIVIANA FALCON CAMA;MARIO LIVAN BARRO ALVAREZ |
分类号 |
A61K38/00;A61K39/00;C07K14/18 |
主分类号 |
A61K38/00 |
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