| 摘要 |
PROBLEM TO BE SOLVED: To efficiently obtain thiotropocin useful as e.g. an antimicrobial agent for fish diseases, anti-fine algal agent, antiallergic agent, by culturing in a medium microbes belonging to Caulobacter and having ability to produce thiotropocin and by gathering the resultant product from the cultured product. SOLUTION: Microbes belonging to Caulobacter and capable of producing thiotropocin [e.g. Caulobacter sp. PK 654 strain (FERM BP-5080)] are inoculated into a medium followed by conducting a shaking culture at 20 deg.C for 5 days at 45rpm to obtain a seed culture fluid which is then inoculated into a medium- contg. triangular flask followed by conducting a culture at 20 deg.C for 5-7 days, and the resultant culture fluid is centrifuged to gather the resultant supernatant which is then filtered and subjected to column chromatography, and the resultant active fraction is recrystallized from methanol, thus efficiently affording the objective thiotropocin useful as an active ingredient of e.g. antimicrobial agents for fish diseases, anti-fine algal agents, antiallergic agents. |
