Method for the detection of creatinine

摘要

Disclosed is a method for the determination of creatinine in urine which involves contacting a urine test sample with a reagent system containing Cu<+2>, a hydroperoxide and an oxidizible dye which provides a detectable response when oxidized in the presence of a pseudoperoxide. The reagent system also includes a buffer to maintain the urine's pH within the range of 6.6 to 8.0 and a chelating agent for the cupric ions to prevent oxidation of the dye in the absence of creatinine.