| 摘要 |
PCT No. PCT/JP94/01384 Sec. 371 Date May 3, 1996 Sec. 102(e) Date May 3, 1996 PCT Filed Aug. 22, 1994 PCT Pub. No. WO95/06135 PCT Pub. Date Mar. 2, 1995The present invention relates to a method of quantitative determination of sodium ions in a sample using beta -galactosidase, wherein the beta -galactosidase reaction is conducted in the presence of at least one chelating agent selected from of 1,2-cyclohexanediamine-N,N,N',N'-tetraacetic acid, ethylenediamine-tetraacetic acid, triethylenetetranine-hexaacetic acid, diethylenetriamine-N,N,N',N'',N''-pentaacetic acid, 1,3 -diminopropan-2-ol-N,N',N'-tetraacetic acid, ethylenediamine-N,N'-dipropionic acid dihydrochloride, ethylenediamine-tetrakis(methylenesulfonic acid), iminodiacetic acid, hydroxyimino-diacetic acid and nitrilotriacetic acid. The method of quantitative determination of sodium ions of the present invention is useful in clinical examinations and the accuracy in the measurement by the method is high.
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