Novel polymeric complexes for the transfection of nucleic acids, with residues causing the destabilisation of cell membranes

摘要

The complex has at least one negatively charged nucleic acid bonded to at least one positively charged polymeric conjugateThe conjugate containing a polylysine formed from monomers having free NH3+ groups, and having at least 10% of the free NH3+ groups substituted by residues which can be protonated in a weakly acid medium causing destabilization of cell membranes.Optionally, some of the free NH3+ groups can be substituted by a molecule with a recognition signal by a cell membrane receptor.The free NH3+ groups of the said polylysine make up at least 30% of the monomers of the polymeric conjugate.The residue that causes the destabilization of cell membrane in weak acid of quinolines of the formula:where R1 is hydrogen, R2 is -(CH2)n13 CO2-H, X is hydrogen or chlorine and n is an integer from 1 to 10.The signal is a simple oside or a disaccharide or peptide.