HETEROLOGOUS ANTIGENS IN LIVE CELL V. CHOLERAE STRAINS

摘要

Disclosed is a V. cholerae cell containing a DNA encoding a fusion protein that includes a heterologous antigenic polypeptide sequence such as a portion of C. difficile toxin A. The fusion protein also includes one of the following: (i) an E. coli hemolysin A subunit (which is coexpressed with sequences encoding hemolysin B and D subunits); (ii) a secretion signal sequence and cholera toxin A2 subunit (which is coexpressed with cholera toxin B subunit); or (iii) cholera toxin B subunit.