发明名称 |
Process for producing cDNA |
摘要 |
The process includes the steps of: a) contacting a liquid containing mRNA with an insoluble support having DNA probes attached thereto via the 5' -terminus thereof whereby the MRNA is hybridized to said probes and hence to said support; b) removing said liquid; and c) adding enzymes and nucleotides in solution whereby the probe functions as a primer to produce single stranded cDNA on the mRNA probe. The probe DNA may be oligo-dT or a specific DNA sequence, such as one that is complementary to a conserved region in a class of mRNA molecules. The insoluble support comprises magnetic particles which are monodisperse polymer particles comprising superparamagnetic iron oxide, a coating to reduce non-specific binding and a substituent for attaching an oligonucleotide.
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申请公布号 |
US5759820(A) |
申请公布日期 |
1998.06.02 |
申请号 |
US19940280133 |
申请日期 |
1994.07.25 |
申请人 |
DYNAL AS |
发明人 |
HORNES, ERIK;KORSNES, LARS |
分类号 |
C12N15/10;C12Q1/68;(IPC1-7):C12P19/34;C12Q1/70;B01J19/08 |
主分类号 |
C12N15/10 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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