| 摘要 |
PROBLEM TO BE SOLVED: To provide an immunoassay method for determining an antigen substance accurately by allowing antigens or antibodies that are related to a reaction to exist as much as possible in an antigen antibody reaction system without causing agglutination inhibition due to the steric hindrance of an insoluble carrier and without conjuring up a non-specific agglutination. SOLUTION: In the immunoassay method, at least two types of different monoclonal antibodies for a specific antigen are carried by an insoluble carrier, are reacted with the antigen in a water-soluble medium, and at least two types of carries with different average particle diameters are used as the insoluble carrier when aggregating the combined substance of the insoluble carrier and the antigen selectively, and the insoluble caries carry each monoclonal antibody. A preferable insoluble carrier is a latex particle. At least two types of latex particles whose average particle diameter ranges from 0.05 to 0.500μm are preferably combined so that the ratio of the average particle diameter of other particle species to that of the minimum particle seed ranges from 3 to 6.
|
