| 摘要 |
PROBLEM TO BE SOLVED: To obtain a new flavonoid glycosidase gene coding UDP-glucose: flavonoid 3,5-O-glucosyl transferase having a specific amino acid sequence, capable of producing a rose, etc., having blue petals by a petal pigment synthase of Gentiana scabra. SOLUTION: This flavonoid glycosidase gene is a new UDP-glucose: flavonoid 3,5-O-glucosyl transferase gene coding an amino acid sequence substantially shown by the formula and concerning biosynthesis of a sugar chain structure of gentiodelphin, which is a petal pigment of Gentiana scabra, and the production of a rose having blue petals, etc., is expected by introducing the gene to a plant body according to a conventional method and expressing the gentiodelphin in a race having no method for regulating coloring of a flower color, especially having no blue petal such as the rose. The gene is obtained by extracting mRNA from the petal of the Gentiana scabra, and performing a PCR reaction of a DNA obtained by performing a reverse transcription reaction of the obtained mRNA by using a primer having a specific sequence of β-saccharide transferase. |
