发明名称 ENZYME ELECTRODE
摘要 PROBLEM TO BE SOLVED: To obtain a reliable and inexpensive enzyme electrode without being influenced by hematocrit in blood samples and with high reliability by dispersing microparticles which contain and enzyme in a water-soluble polymer into the matrix of an electroconductive microparticle layer. SOLUTION: This electrode 1, which is not influenced by blood hematocrit by keeping the ratio of microparticles 13 which contact with erythrocytes 2 in a blood sample 3 low, is obtained by (1) forming an electroconductive layer 11 such as a lead wire on an insulated support 10 such as a glass epoxy substrate using carbon paste and silver paste, (2) coating on it a mixed/dispersed liquid that was prepared by adding a phosphate buffer that contains an enzyme (e.g. peroxidase) and a water-soluble polymer (e.g. dextran), to a hydrophobic solvent that was mixed/dispersed with electrocoductive microparticles, and by blending with a mixer, and (3) drying the electrode at a temperature (20-60 deg.C) that does not inactivate the enzyme, wherein the microparticles 13 which comprise the water-soluble polymer containing the enzyme are dispersed in voids in the matrix of an electroconductive microparticle layer 12.
申请公布号 JPH10113200(A) 申请公布日期 1998.05.06
申请号 JP19960289121 申请日期 1996.10.11
申请人 IATRON LAB INC 发明人 TSURUTA KYOKO;OGASAWARA MINORU;AIZAWA MASUO
分类号 G01N27/327;C12Q1/25;C12Q1/54 主分类号 G01N27/327
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