发明名称 |
Method for establishing a tumor-cell line by preparing single-cell suspension of tumor cells from tumor biopsies |
摘要 |
The present invention provides a novel method for the establishment of tumorigenic cell lines from biopsies of human or animal tumors. The method includes preparing a single-cell suspension of a tumor cells by injecting cell culture medium into an isolated tumor or a portion thereof to flush out the tumor cells. The tumor or portion thereof may be in a cell culture maintenance medium. The step of injecting cell culture medium into the isolated tumor or portion thereof involves piercing the tumor or portion thereof with a needle to inject the cell culture medium and flush out the tumor cells. The steps of piercing the tumor or portion thereof with a needle and injecting the cell culture medium to flush out the tumor cells preferably are repeated until the single-cell suspension has a particular cell density. The method also includes establishing a primary tumor cell culture by growing the cells from the single-cell suspension in a cell culture medium.
|
申请公布号 |
US5744363(A) |
申请公布日期 |
1998.04.28 |
申请号 |
US19960764571 |
申请日期 |
1996.12.13 |
申请人 |
NATIONAL INSTITUTE OF IMMUNOLOGY |
发明人 |
MUKHERJEE, RAMA;JAGGI, MANU |
分类号 |
A61K38/08;A61K38/10;C12N5/09;(IPC1-7):C12N5/00 |
主分类号 |
A61K38/08 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|