| 摘要 |
A means (DNA identification card) and a method for identification of the sequence of DNA nucleotides by laser spectroscopy. A DNA identification card is a solid means onto/into which the nucleotides or their groups cut off from the DNA molecule are carried and fixed with the same sequence as in the DNA and where the nucleotides or their groups are placed from one another at distances exceeding the wavelength of the light exciting the fluorescence of marker molecules or atoms. The nucleotides or their groups are identified and their sequences determined on the DNA identification card by the fluorescence spectrum of marked nucleotides using method of single molecular detection (SMD) or single molecular spectroscopy (SMS). High spectral resolution is achieved by cooling the DNA identification card to temperatures below 10 K and using specific markers which carry at these temperatures with them into the fluorescence excitation spectra narrow and intense zerophonon lines. |
