There is provided an assay to determine whether an animal has an allele for or exhibits muscle fibre composition characteristic of a Duroc pig. The assay comprises obtaining a tissue sample from said animal and subjecting said sample to genetic analysis and/or obtaining a muscle sample from said animal and determining by histochemical or immunochemical analysis whether said sample exhibits phenotypic traits indicative of said allele(s) and/or shows a muscle composition characterisitc to the Duroc pig. Suitable physical traits include the following: i. the percentage frequency of SO fibres present in said muscle; and/or ii. the number of SO fibres present per cluster; and/or iii. the number of muscle fibres present per cluster; and/or iv. the level of m calpain present per unit muscle; and/or v. the level of .mu. calpain present per unit muscle. The assay can be used to select animals having Duroc muscle type for breeding or for meat production. Anti-calpain antibodies are especially useful and may be employed as part of an ELISA style assay.
申请公布号
CA2267347(A1)
申请公布日期
1998.04.16
申请号
CA19972267347
申请日期
1997.10.07
申请人
MEAT AND LIVESTOCK COMMISSION
发明人
STEVEN, JOHN;WARKUP, CHRISTOPHER CHARLES;MALTIN, CHARLOTTE ANNE