METHOD OF TARGETING MALIGNANT CELLS USING AN E2F RESPONSIVE PROMOTER

摘要

A method of selectively expressing a gene in a malignant as opposed to a non-malignant cell is taught. This permits one to selectively express proteins that would be deletorious to normal cells with minimal harm. The method involves the use of a nucleic acid cassette having an E2F responsive promoter operably linked to a gene of interest, which encodes either a positive or negative potentiator such as antibodies, dominant negative mutants, suicide genes, antisense RNA, ribozymes and cytotoxic agents.