| 摘要 |
Novel acid-stable and thermo-stable enzymes having 'alpha'-1,4 hydrolytic activity and a 'alpha'-1,6 hydrolytic activity which are derived from strains of the genus Sulfolobus. These enzymes are capable of expressing high levels of 'alpha'-1,4 hydrolytic activity, including the maximum 'alpha'-1,4 hydrolytic activity thereof, at highly acidic pHs of between about 2.5 and about 4.5. These 'alpha'-amylases are further capable of expressing high levels of 'alpha'-1,4 hydrolytic activity, including the maximum 'alpha'-1,4 hydrolytic activity thereof, at high temperatures of between about 90 degree C and about 120 degree C. Particularly disclosed herein are such enzymes which are derived from strains of the species S. acidocaldarius and, in particular, Sulfolobus acidocaldarius DSM 639. Modified starch degradation (liquefaction and saccharification) processes using these novel enzymes are also disclosed herein. |
