| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for a solid phase synthesis of an oligonucleotide providing an improved method for removing a protecting group from the oligonucleotide bound to a solid supporting materials. SOLUTION: This method for a solid phase synthesis of an oligonucleotide, is (a) to synthesize nucleic acids consectively on a solid supporting material according to a known method, where amino groups out of ring existing in the nucleic acid base are protected with cyclic diacyl groups, (b) remove a protecting group from the oligonucleotide bound to the solid supporting material by removing a phosphate protecting group with a non-nucleophilic strong base, and (c) separate the protecting group-removed oligonucleotide from the solid supporting material. In this case, the protecting group is removed from the oligonucleotide bound to the solid supporting material by a suitable organic solvent in the presence of the non-nucleophilic strong base.</p> |
