发明名称 |
CELL-BASED DRUG SCREENS FOR REGULATORS OF GENE EXPRESSION |
摘要 |
The invention provides methods and compositions for screening for pharmacological agents which regulate gene expression in mammals. An exemplary assay involves (a) contacting a mammalian cell comprising a knock-in mutant of a targeted native allele encoding a reporter of gene expression, as shown in the figure, wherein the expression of the reporter is under the control of the gene expression regulatory sequences of the native allele, with a candidate agent under conditions whereby but for the presence of the agent, the reporter is expressed at a first expression level; and (b) measuring the expression of the reporter to obtain a second expression level, wherein a difference between the first and second expressions levels indicates that the candidate agent modulates gene expression.
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申请公布号 |
WO9810059(A1) |
申请公布日期 |
1998.03.12 |
申请号 |
WO1997US15454 |
申请日期 |
1997.09.02 |
申请人 |
TULARIK, INC. |
发明人 |
SHAN, BEI;LEARNED, R., MARC;AMARAL, M., CATHERINE;MCKNIGHT, STEVEN, L.;DE LA BROUSSE, FABIENNE, C.;CHEN, JIN-LONG |
分类号 |
C12N15/09;C12N5/10;C12Q1/66;C12Q1/68;G01N33/50;(IPC1-7):C12N5/10;C12N15/10;C12N15/63;C12N15/64;C12N15/85 |
主分类号 |
C12N15/09 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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