STRAIN OF BACTERIUM DELEYA AQUAMARINA - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND CLEAVING NUCLEOTIDE SEQUENCE 5'-GTGCAC-3'

摘要

FIELD: biotechnology, biochemistry, genetic engineering. SUBSTANCE: strain Deleya aquamarina - a producer of restriction endonuclease DagI is isolated from sea-water as a result of search and screening restrictase producers. Strain provides preparing restrictase that recognizes and cleaves nucleotide sequence 5'-GTGCAC-3'. Enzyme yield is 18000 U/g crude biomass, specific activity of the end enzyme is 20000 U/ml. Restictase DagI is an isoschizomer of restrictase <EMI ID=0.347 HE=3 WI=24 TI=CHI> and can replace the latter in all genetic-engineering works. Invention can be used for research of primary DNA structure and mapping of different genomes. EFFECT: strain-producer indicated above, increased activity and yield of enzyme. 1 dwgx