发明名称 |
Mechanism-based screen for retinoid X receptor agonists and antagonists |
摘要 |
A method is described for screening retinoid X receptor agonists or antagonists using a retinoid X receptor expressed by a yeast expression system to screen a compound having a retinoid X receptor agonist or antagonist activity. Another screen is described for detecting a compound having retinoid X receptor agonist or antagonist activity by: (1) providing a yeast strain which expresses the retinoic acid receptor and activates a reporter plasmid containing apolipoprotein AI gene site A or a mutated variant thereof; (2) incubating the compound in suitable media and a colorless chromogenic substrate; and (3) examining the media for development of color. Mutated variants of apolipoprotein AI gene site A which respond selectively to the retinoid X receptor or respond to receptors other than RXR alpha are also described.
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申请公布号 |
US5714595(A) |
申请公布日期 |
1998.02.03 |
申请号 |
US19950469120 |
申请日期 |
1995.06.06 |
申请人 |
AMERICAN CYANAMID COMPANY |
发明人 |
MAK, PAUL;KARATHANASIS, SOTIRIOS K. |
分类号 |
C07K14/705;C07K14/775;C12N1/21;C12N15/09;C12Q1/02;C12Q1/68;C12R1/19;G01N33/566;(IPC1-7):C07H21/04 |
主分类号 |
C07K14/705 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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